› 3cdg

X-ray diffraction
3.4Å resolution

Human CD94/NKG2A in complex with HLA-E

Released:
DOI: 10.2210/pdb3cdg/pdb
PDB entry 3cdg coloured by chain, front view.
PDB entry 3cdg coloured by chain, side view.
PDB entry 3cdg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence.
Petrie EJ, Clements CS, Lin J, Sullivan LC, Johnson D, Huyton T, Heroux A, Hoare HL, Beddoe T, Reid HH, Wilce MC, Brooks AG, Rossjohn J
J Exp Med 205 725-35 (2008)
PMID: 18332182

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146744 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, C
Molecule details ›
Chains: A, C
Length: 273 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13747 (Residues: 23-295; Coverage: 81%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Natural killer cells antigen CD94 Chains: E, J
Molecule details ›
Chains: E, J
Length: 123 amino acids
Theoretical weight: 14.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13241 (Residues: 57-179; Coverage: 69%)
Gene names: CD94, KLRD1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
NKG2-A/NKG2-B type II integral membrane protein Chains: F, K
Molecule details ›
Chains: F, K
Length: 120 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26715 (Residues: 113-232; Coverage: 52%)
Gene names: KLRC1, NKG2A
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Soluble HLA class I histocompatibility antigen, alpha chain G Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P17693 (Residues: 3-11; Coverage: 0%)
Gene names: HLA-6.0, HLA-G, HLAG

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4132
Unit cell:
a: 345.201Å b: 345.201Å c: 345.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

49 review citations

Variable NK cell receptors and their MHC class I ligands in immunity, reproduction and human evolution.
Parham et al. (2013)
48 more

21 mentions without citation

Structural basis for recognition of cellular and viral ligands by NK cell receptors.
Li et al. (2014)
20 more