› 3cf0

X-ray diffraction
3Å resolution

Structure of D2 subdomain of P97/VCP in complex with ADP

Released:
DOI: 10.2210/pdb3cf0/pdb
PDB entry 3cf0 coloured by chain, front view.
PDB entry 3cf0 coloured by chain, side view.
PDB entry 3cf0 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Improved structures of full-length p97, an AAA ATPase: implications for mechanisms of nucleotide-dependent conformational change.
Davies JM, Brunger AT, Weis WI
Structure 16 715-26 (2008)
PMID: 18462676

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transitional endoplasmic reticulum ATPase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 301 amino acids
Theoretical weight: 33.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01853 (Residues: 463-763; Coverage: 37%)
Gene name: Vcp
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 14 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 173.317Å b: 167.223Å c: 209.465Å
α: 90° β: 112.29° γ: 90°
R-values:
R R work R free
0.244 0.243 0.262
Expression system: Escherichia coli

Quick links

Citations

40 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
39 more

8 mentions without citation

Structure and function of the AAA+ ATPase p97/Cdc48p.
Xia et al. (2016)
7 more