› 3cf1

X-ray diffraction
4.4Å resolution

Structure of P97/vcp in complex with ADP/ADP.alfx

Released:
DOI: 10.2210/pdb3cf1/pdb
PDB entry 3cf1 coloured by chain, front view.
PDB entry 3cf1 coloured by chain, side view.
PDB entry 3cf1 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Improved structures of full-length p97, an AAA ATPase: implications for mechanisms of nucleotide-dependent conformational change.
Davies JM, Brunger AT, Weis WI
Structure 16 715-26 (2008)
PMID: 18462676

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transitional endoplasmic reticulum ATPase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 806 amino acids
Theoretical weight: 89.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01853 (Residues: 1-806; Coverage: 100%)
Gene name: Vcp
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 6 x ADP
Ligand AF3 3 x AF3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I222
Unit cell:
a: 162.66Å b: 178.02Å c: 321.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.286
Expression system: Escherichia coli

Quick links

Citations

38 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
37 more

31 mentions without citation

Structure and function of the AAA+ ATPase p97/Cdc48p.
Xia et al. (2016)
30 more