› 3cf2

X-ray diffraction
3.5Å resolution

Structure of P97/vcp in complex with ADP/AMP-PNP

Released:
DOI: 10.2210/pdb3cf2/pdb
PDB entry 3cf2 coloured by chain, front view.
PDB entry 3cf2 coloured by chain, side view.
PDB entry 3cf2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Improved structures of full-length p97, an AAA ATPase: implications for mechanisms of nucleotide-dependent conformational change.
Davies JM, Brunger AT, Weis WI
Structure 16 715-26 (2008)
PMID: 18462676

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169640 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transitional endoplasmic reticulum ATPase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 806 amino acids
Theoretical weight: 89.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01853 (Residues: 1-806; Coverage: 100%)
Gene name: Vcp
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 4 x ADP
Ligand ANP 4 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3
Unit cell:
a: 144.9Å b: 144.9Å c: 164.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.271 0.271 0.285
Expression system: Escherichia coli

Quick links

Citations

37 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
36 more

20 mentions without citation

Structure and function of the AAA+ ATPase p97/Cdc48p.
Xia et al. (2016)
19 more