› 3cfd

X-ray diffraction
2.5Å resolution

Purple-fluorescent antibody EP2-25C10 in complex with its stilbene hapten

Released:
DOI: 10.2210/pdb3cfd/pdb
PDB entry 3cfd coloured by chain, front view.
PDB entry 3cfd coloured by chain, side view.
PDB entry 3cfd coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.
Debler EW, Kaufmann GF, Meijler MM, Heine A, Mee JM, Pljevaljcic G, Di Bilio AJ, Schultz PG, Millar DP, Janda KD, Wilson IA, Gray HB, Lerner RA
Science 319 1232-5 (2008)
PMID: 18309081

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213230 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PURPLE-FLUORESCENT ANTIBODY EP2-25C10-KAPPA LIGHT CHAIN Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.61 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
PURPLE-FLUORESCENT ANTIBODY EP2-25C10-IGG2B HEAVY CHAIN Chains: B, H
Molecule details ›
Chains: B, H
Length: 220 amino acids
Theoretical weight: 23.81 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SPB 2 x SPB
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 59.99Å b: 72.48Å c: 77.23Å
α: 91.98° β: 106.11° γ: 114.15°
R-values:
R R work R free
0.212 0.211 0.243

Quick links

Citations

2 review citations

Aggregation-induced emission.
Hong et al. (2011)
1 more

1 mention without citation

Structural Analysis of Anti-Hapten Antibodies to Identify Long-Range Structural Movements Induced by Hapten Binding.
Al Qaraghuli et al. (2021)