› 3ch8

X-ray diffraction
1.9Å resolution

The crystal structure of PDZ-Fibronectin fusion protein

Released:
DOI: 10.2210/pdb3ch8/pdb
PDB entry 3ch8 coloured by chain, front view.
PDB entry 3ch8 coloured by chain, side view.
PDB entry 3ch8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for exquisite specificity of affinity clamps, synthetic binding proteins generated through directed domain-interface evolution.
Huang J, Makabe K, Biancalana M, Koide A, Koide S
J Mol Biol 392 1221-31 (2009)
PMID: 19646997

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163458 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Erbin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96RT1 (Residues: 1329-1410; Coverage: 6%)
Gene names: ERBB2IP, ERBIN, KIAA1225, LAP2
Sequence domains: PDZ domain
Structure domains:
C-terminal octapeptide from protein ARVCF Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 927 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P32
Unit cell:
a: 69.008Å b: 69.008Å c: 46.706Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.201 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Superglue from bacteria: unbreakable bridges for protein nanotechnology.
Veggiani et al. (2014)
9 more

9 mentions without citation

Generation of new protein functions by nonhomologous combinations and rearrangements of domains and modules.
Koide S. (2009)
8 more