› 3chw

X-ray diffraction
2.3Å resolution

Complex of Dictyostelium discoideum Actin with Profilin and the Last Poly-Pro of Human VASP

Released:
DOI: 10.2210/pdb3chw/pdb
PDB entry 3chw coloured by chain, front view.
PDB entry 3chw coloured by chain, side view.
PDB entry 3chw coloured by chain, top view.
Source organisms:
Primary publication:
Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.
Baek K, Liu X, Ferron F, Shu S, Korn ED, Dominguez R
Proc Natl Acad Sci U S A 105 11748-53 (2008)
PMID: 18689676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-139727 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major actin Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.66 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P07830 (Residues: 2-376; Coverage: 100%)
Gene names: DDB_G0269234, DDB_G0272248, DDB_G0272520, DDB_G0274129, DDB_G0274133, DDB_G0274135, DDB_G0274137, DDB_G0274285, DDB_G0274561, DDB_G0274599, DDB_G0274601, DDB_G0274727, DDB_G0280545, DDB_G0288879, DDB_G0289005, DDB_G0289553, DDB_G0289663, act1, act11, act12, act13, act14, act15, act16, act19, act1a, act2, act2-1, act20, act21, act4, act5, act6, act7, act8, act9, actA1, actA8, actB1, actM6
Sequence domains: Actin
Structure domains:
Profilin-1 Chain: P
Molecule details ›
Chain: P
Length: 139 amino acids
Theoretical weight: 14.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07737 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic
Vasodilator-stimulated phosphoprotein Chain: V
Molecule details ›
Chain: V
Length: 16 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P50552 (Residues: 199-214; Coverage: 4%)
Gene name: VASP

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 38.037Å b: 76.183Å c: 180.822Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.157 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Tropomodulins and Leiomodins: Actin Pointed End Caps and Nucleators in Muscles.
Fowler et al. (2017)
6 more

9 mentions without citation

Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.
Baek et al. (2008)
8 more