› 3ci5

X-ray diffraction
1.7Å resolution

Complex of Phosphorylated Dictyostelium Discoideum Actin with Gelsolin

Released:
DOI: 10.2210/pdb3ci5/pdb
PDB entry 3ci5 coloured by chain, front view.
PDB entry 3ci5 coloured by chain, side view.
PDB entry 3ci5 coloured by chain, top view.
Source organisms:
Primary publication:
Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.
Baek K, Liu X, Ferron F, Shu S, Korn ED, Dominguez R
Proc Natl Acad Sci U S A 105 11748-53 (2008)
PMID: 18689676

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Major actin Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.74 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P07830 (Residues: 2-376; Coverage: 100%)
Gene names: DDB_G0269234, DDB_G0272248, DDB_G0272520, DDB_G0274129, DDB_G0274133, DDB_G0274135, DDB_G0274137, DDB_G0274285, DDB_G0274561, DDB_G0274599, DDB_G0274601, DDB_G0274727, DDB_G0280545, DDB_G0288879, DDB_G0289005, DDB_G0289553, DDB_G0289663, act1, act11, act12, act13, act14, act15, act16, act19, act1a, act2, act2-1, act20, act21, act4, act5, act6, act7, act8, act9, actA1, actA8, actB1, actM6
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 126 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06396 (Residues: 52-176; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand SO4 10 x SO4
Ligand ATP 1 x ATP
Ligand GOL 5 x GOL
Ligand CA 1 x CA
2 modified residues:
Ligand PTR 1 x PTR
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: C2
Unit cell:
a: 178.423Å b: 69.045Å c: 56.54Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.143 0.141 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Tropomodulins and Leiomodins: Actin Pointed End Caps and Nucleators in Muscles.
Fowler et al. (2017)
6 more

9 mentions without citation

Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.
Baek et al. (2008)
8 more