› 3cjb

X-ray diffraction
3.21Å resolution

Actin dimer cross-linked by V. cholerae MARTX toxin and complexed with Gelsolin-segment 1

Released:
DOI: 10.2210/pdb3cjb/pdb
PDB entry 3cjb coloured by chain, front view.
PDB entry 3cjb coloured by chain, side view.
PDB entry 3cjb coloured by chain, top view.
Source organisms:
Primary publication:
Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.
Kudryashov DS, Durer ZA, Ytterberg AJ, Sawaya MR, Pashkov I, Prochazkova K, Yeates TO, Loo RR, Loo JA, Satchell KJ, Reisler E
Proc Natl Acad Sci U S A 105 18537-42 (2008)
PMID: 19015515

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139014 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 125 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06396 (Residues: 52-176; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 56.696Å b: 69.54Å c: 182.106Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.245 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

RTX proteins: a highly diverse family secreted by a common mechanism.
Linhartová et al. (2010)
12 more

3 mentions without citation

Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.
Kudryashov et al. (2008)
2 more