› 3cmq

X-ray diffraction
2.2Å resolution

Crystal structure of human mitochondrial phenylalanine tRNA synthetase

Released:
DOI: 10.2210/pdb3cmq/pdb
PDB entry 3cmq coloured by chain, front view.
PDB entry 3cmq coloured by chain, side view.
PDB entry 3cmq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The tRNA-induced conformational activation of human mitochondrial phenylalanyl-tRNA synthetase.
Klipcan L, Levin I, Kessler N, Moor N, Finarov I, Safro M
Structure 16 1095-104 (2008)
PMID: 18611382

Function and Biology Details

Reaction catalysed: 
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 48.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95363 (Residues: 38-451; Coverage: 92%)
Gene names: FARS1, FARS2, HSPC320
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand FA5 1 x FA5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 54.888Å b: 90.168Å c: 95.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.192 0.242
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Recent Advances in Mitochondrial Aminoacyl-tRNA Synthetases and Disease.
Sissler et al. (2017)
3 more

6 mentions without citation

Eukaryotic cytosolic and mitochondrial phenylalanyl-tRNA synthetases catalyze the charging of tRNA with the meta-tyrosine.
Klipcan et al. (2009)
5 more