› 3coa

X-ray diffraction
2.2Å resolution

Crystal Structure of FoxO1 DBD Bound to IRE DNA

Released:
DOI: 10.2210/pdb3coa/pdb
PDB entry 3coa coloured by chain, front view.
PDB entry 3coa coloured by chain, side view.
PDB entry 3coa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition by FoxO1 and its regulation by posttranslational modification.
Brent MM, Anand R, Marmorstein R
Structure 16 1407-16 (2008)
PMID: 18786403

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116148 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein O1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 117 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12778 (Residues: 151-266; Coverage: 18%)
Gene names: FKHR, FOXO1, FOXO1A
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*DTP*DGP*DGP*DTP*DTP*DTP*DGP*DTP*DTP*DTP*DTP*DGP*DCP*DTP*DTP*DG)-3') Chains: A, D
Molecule details ›
Chains: A, D
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*DCP*DAP*DAP*DGP*DCP*DAP*DAP*DAP*DAP*DCP*DAP*DAP*DAP*DCP*DCP*DA)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21212
Unit cell:
a: 76.062Å b: 102.432Å c: 65.446Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

38 review citations

FOXO transcription factors throughout T cell biology.
Hedrick et al. (2012)
37 more

9 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
8 more