› 3cqq

X-ray diffraction
1.9Å resolution

Human SOD1 G85R Variant, Structure II

Released:
DOI: 10.2210/pdb3cqq/pdb
PDB entry 3cqq coloured by chain, front view.
PDB entry 3cqq coloured by chain, side view.
PDB entry 3cqq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the G85R variant of SOD1 in familial amyotrophic lateral sclerosis.
Cao X, Antonyuk SV, Seetharaman SV, Whitson LJ, Taylor AB, Holloway SP, Strange RW, Doucette PA, Valentine JS, Tiwari A, Hayward LJ, Padua S, Cohlberg JA, Hasnain SS, Hart PJ
J Biol Chem 283 16169-77 (2008)
PMID: 18378676

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 15.94 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00441 (Residues: 2-154; Coverage: 99%)
Gene name: SOD1
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand ACE 1 x ACE
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: P212121
Unit cell:
a: 40.038Å b: 58.442Å c: 104.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.24
Expression system: Saccharomyces cerevisiae

Quick links

Citations

8 review citations

Amyloid formation by globular proteins under native conditions.
Chiti et al. (2009)
7 more

4 mentions without citation

Structural Properties and Interaction Partners of Familial ALS-Associated SOD1 Mutants.
Huai et al. (2019)
3 more