› 3csy

X-ray diffraction
3.4Å resolution

Crystal structure of the trimeric prefusion Ebola virus glycoprotein in complex with a neutralizing antibody from a human survivor

Released:
DOI: 10.2210/pdb3csy/pdb
PDB entry 3csy coloured by chain, front view.
PDB entry 3csy coloured by chain, side view.
PDB entry 3csy coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor.
Lee JE, Fusco ML, Hessell AJ, Oswald WB, Burton DR, Saphire EO
Nature 454 177-82 (2008)
PMID: 18615077

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211919 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (8 distinct):
Fab KZ52 heavy chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 226 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab KZ52 light chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 217 amino acids
Theoretical weight: 23.94 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
GP1 Chains: I, K, M, O
Molecule details ›
Chains: I, K, M, O
Length: 334 amino acids
Theoretical weight: 37.03 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 32-336; Coverage: 47%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
GP2 Chains: J, L, N, P
Molecule details ›
Chains: J, L, N, P
Length: 131 amino acids
Theoretical weight: 14.78 KDa
Source organism: Ebola virus - Mayinga, Zaire, 1976
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 502-632; Coverage: 20%)
Gene name: GP

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R32
Unit cell:
a: 273.71Å b: 273.71Å c: 409.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.261 0.302
Expression systems:
  • Cricetulus griseus
  • Homo sapiens

Quick links

Citations

79 review citations

Viral membrane fusion.
Harrison SC. (2015)
78 more

92 mentions without citation

Filovirus entry: a novelty in the viral fusion world.
Hunt et al. (2012)
91 more