› 3ctr

X-ray diffraction
2.1Å resolution

Crystal structure of the RRM-domain of the poly(A)-specific ribonuclease PARN bound to m7GTP

Released:
DOI: 10.2210/pdb3ctr/pdb
PDB entry 3ctr coloured by chain, front view.
PDB entry 3ctr coloured by chain, side view.
PDB entry 3ctr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m(7)G-cap-binding mode.
Monecke T, Schell S, Dickmanns A, Ficner R
J Mol Biol 382 827-34 (2008)
PMID: 18694759

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-131933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A)-specific ribonuclease PARN Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95453 (Residues: 445-540; Coverage: 15%)
Gene names: DAN, PARN
Sequence domains: RNA binding domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand MGP 1 x MGP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A, BESSY BEAMLINE 14.2
Spacegroup: I4122
Unit cell:
a: 81.107Å b: 81.107Å c: 78.058Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Poly(A)-specific ribonuclease (PARN): an allosterically regulated, processive and mRNA cap-interacting deadenylase.
Virtanen et al. (2013)
7 more

6 mentions without citation

NCBP3: A Multifaceted Adaptive Regulator of Gene Expression.
Rambout et al. (2021)
5 more