› 3cuk

X-ray diffraction
2.49Å resolution

Crystal structure of human D-amino acid oxidase: bound to an inhibitor

Released:
DOI: 10.2210/pdb3cuk/pdb
PDB entry 3cuk coloured by chain, front view.
PDB entry 3cuk coloured by chain, side view.
PDB entry 3cuk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The discovery of fused pyrrole carboxylic acids as novel, potent D-amino acid oxidase (DAO) inhibitors.
Sparey T, Abeywickrema P, Almond S, Brandon N, Byrne N, Campbell A, Hutson PH, Jacobson M, Jones B, Munshi S, Pascarella D, Pike A, Prasad GS, Sachs N, Sakatis M, Sardana V, Venkatraman S, Young MB
Bioorg Med Chem Lett 18 3386-91 (2008)
PMID: 18455394

Function and Biology Details

Reaction catalysed: 
A D-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-amino-acid oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 347 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14920 (Residues: 1-347; Coverage: 100%)
Gene names: DAMOX, DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:
Ligand 4P5 4 x 4P5
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 187.729Å b: 51.145Å c: 153.294Å
α: 90° β: 110.45° γ: 90°
R-values:
R R work R free
0.243 0.239 0.328
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The neurobiology of D-amino acid oxidase and its involvement in schizophrenia.
Verrall et al. (2010)
4 more

2 mentions without citation

Competitive Inhibitors Unveil Structure/Function Relationships in Human D-Amino Acid Oxidase.
Molla G. (2017)
1 more