› 3d10

X-ray diffraction
1.65Å resolution

Crystal Structure of S100B in the Calcium and Zinc Loaded State at pH 10.0

Released:
DOI: 10.2210/pdb3d10/pdb
PDB entry 3d10 coloured by chain, front view.
PDB entry 3d10 coloured by chain, side view.
PDB entry 3d10 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structures of human S100B in the zinc- and calcium-loaded state at three pH values reveal zinc ligand swapping.
Ostendorp T, Diez J, Heizmann CW, Fritz G
Biochim Biophys Acta 1813 1083-91 (2011)
PMID: 20950652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein S100-B Chains: A, B
Molecule details ›
Chains: A, B
Length: 92 amino acids
Theoretical weight: 10.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04271 (Residues: 1-92; Coverage: 100%)
Gene name: S100B
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand ZN 2 x ZN
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 34.75Å b: 58.18Å c: 47.85Å
α: 90° β: 111.08° γ: 90°
R-values:
R R work R free
0.212 0.209 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Functions of S100 proteins.
Donato et al. (2013)
5 more

20 mentions without citation

EVI1 dysregulation: impact on biology and therapy of myeloid malignancies.
Birdwell et al. (2021)
19 more