› 3d25

X-ray diffraction
1.3Å resolution

Crystal structure of HA-1 minor histocompatibility antigen bound to human class I MHC HLA-A2

Released:
DOI: 10.2210/pdb3d25/pdb
PDB entry 3d25 coloured by chain, front view.
PDB entry 3d25 coloured by chain, side view.
PDB entry 3d25 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Secondary anchor polymorphism in the HA-1 minor histocompatibility antigen critically affects MHC stability and TCR recognition.
Nicholls S, Piper KP, Mohammed F, Dafforn TR, Tenzer S, Salim M, Mahendra P, Craddock C, van Endert P, Schild H, Cobbold M, Engelhard VH, Moss PA, Willcox BE
Proc Natl Acad Sci U S A 106 3889-94 (2009)
PMID: 19234124

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137823 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 22-119; Coverage: 99%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Minor histocompatibility antigen HA-1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q92619 (Residues: 137-145; Coverage: 1%)
Gene names: ARHGAP45, HMHA1, KIAA0223

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 53.17Å b: 80.98Å c: 56.26Å
α: 90° β: 112.32° γ: 90°
R-values:
R R work R free
0.169 not available 0.204
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Exploiting T cells specific for human minor histocompatibility antigens for therapy of leukemia.
Bleakley et al. (2011)
7 more

10 mentions without citation

Effect of MHC and non-MHC donor/recipient genetic disparity on the outcome of allogeneic HCT.
Warren et al. (2012)
9 more