› 3d32

X-ray diffraction
1.3Å resolution

Complex of GABA(A) receptor-associated protein (GABARAP) with a synthetic peptide

Released:
DOI: 10.2210/pdb3d32/pdb
PDB entry 3d32 coloured by chain, front view.
PDB entry 3d32 coloured by chain, side view.
PDB entry 3d32 coloured by chain, top view.
Source organisms:
Primary publication:
Ligand binding mode of GABAA receptor-associated protein.
Weiergräber OH, Stangler T, Thielmann Y, Mohrlüder J, Wiesehan K, Willbold D
J Mol Biol 381 1320-31 (2008)
PMID: 18638487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131850 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gamma-aminobutyric acid receptor-associated protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 14.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O95166 (Residues: 1-117; Coverage: 100%)
Gene names: FLC3B, GABARAP, HT004
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
K1 peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 amino acids
Theoretical weight: 1.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand CL 8 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 129.287Å b: 35.415Å c: 58.209Å
α: 90° β: 100.59° γ: 90°
R-values:
R R work R free
0.178 0.177 0.195
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

6 review citations

Dynamic regulation of macroautophagy by distinctive ubiquitin-like proteins.
Klionsky et al. (2014)
5 more

8 mentions without citation

Benchmarking of different molecular docking methods for protein-peptide docking.
Agrawal et al. (2019)
7 more