› 3d8c

X-ray diffraction
2.1Å resolution

Factor inhibiting HIF-1 alpha D201G mutant in complex with ZN(II), alpha-ketoglutarate and HIF-1 alpha 19mer

Released:
DOI: 10.2210/pdb3d8c/pdb
PDB entry 3d8c coloured by chain, front view.
PDB entry 3d8c coloured by chain, side view.
PDB entry 3d8c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evidence that two enzyme-derived histidine ligands are sufficient for iron binding and catalysis by factor inhibiting HIF (FIH).
Hewitson KS, Holmes SL, Ehrismann D, Hardy AP, Chowdhury R, Schofield CJ, McDonough MA
J Biol Chem 283 25971-8 (2008)
PMID: 18611856

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163475 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWT6 (Residues: 11-349; Coverage: 97%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:
HYPOXIA-INDUCIBLE FACTOR 1 ALPHA Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.05 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 2 x SO4
Ligand AKG 1 x AKG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 86.427Å b: 86.427Å c: 148.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.256
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

Inhibition of 2-oxoglutarate dependent oxygenases.
Rose et al. (2011)
7 more

5 mentions without citation

Evidence that two enzyme-derived histidine ligands are sufficient for iron binding and catalysis by factor inhibiting HIF (FIH).
Hewitson et al. (2008)
4 more