› 3dae

X-ray diffraction
2.9Å resolution

Crystal structure of phosphorylated SNF1 kinase domain

Released:
DOI: 10.2210/pdb3dae/pdb
PDB entry 3dae coloured by chain, front view.
PDB entry 3dae coloured by chain, side view.
PDB entry 3dae coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insight into the autoinhibition mechanism of AMP-activated protein kinase.
Chen L, Jiao ZH, Zheng LS, Zhang YY, Xie ST, Wang ZX, Wu JW
Nature 459 1146-9 (2009)
PMID: 19474788

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-139223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbon catabolite-derepressing protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 32.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06782 (Residues: 41-315; Coverage: 43%)
Gene names: CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P212121
Unit cell:
a: 74.607Å b: 72.37Å c: 112.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

48 review citations

AMPK: a nutrient and energy sensor that maintains energy homeostasis.
Hardie et al. (2012)
47 more

3 mentions without citation

Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases.
Ng et al. (2011)
2 more