› 3dax

X-ray diffraction
2.15Å resolution

Crystal structure of human CYP7A1

Released:
DOI: 10.2210/pdb3dax/pdb
PDB entry 3dax coloured by chain, front view.
PDB entry 3dax coloured by chain, side view.
PDB entry 3dax coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Strushkevich NV, Tempel W, Dombrovski L, Dong A, Loppnau P, Arrowsmith CH, Edwards AM, Bountra C, Wilkstrom M, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
Cholesterol + [reduced NADPH--hemoprotein reductase] + O(2) = 7-alpha-hydroxycholesterol + [oxidized NADPH--hemoprotein reductase] + H(2)O
(24S)-cholest-5-ene-3-beta,24-diol + [reduced NADPH--hemoprotein reductase] + O(2) = (24S)-cholest-5-ene-3-beta,7-alpha,24-triol + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 7A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 56.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22680 (Residues: 25-503; Coverage: 95%)
Gene names: CYP7, CYP7A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand UNX 2 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, null
Spacegroup: P1
Unit cell:
a: 55.339Å b: 80.159Å c: 84.937Å
α: 64.36° β: 75.23° γ: 72.17°
R-values:
R R work R free
0.19 0.189 0.228
Expression system: Escherichia coli

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