› 3dfx

X-ray diffraction
2.7Å resolution

Opposite GATA DNA binding

Released:
DOI: 10.2210/pdb3dfx/pdb
PDB entry 3dfx coloured by chain, front view.
PDB entry 3dfx coloured by chain, side view.
PDB entry 3dfx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structures of multiple GATA zinc fingers bound to DNA reveal new insights into DNA recognition and self-association by GATA.
Bates DL, Chen Y, Kim G, Guo L, Chen L
J Mol Biol 381 1292-306 (2008)
PMID: 18621058

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116176 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Trans-acting T-cell-specific transcription factor GATA-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23772 (Residues: 308-370; Coverage: 14%)
Gene names: Gata-3, Gata3
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*DTP*DTP*DGP*DAP*DTP*DAP*DAP*DAP*DTP*DCP*DAP*DGP*DAP*DGP*DAP*DTP*DAP*DAP*DCP*DC)-3') Chain: X
Molecule details ›
Chain: X
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*DAP*DAP*DGP*DGP*DTP*DTP*DAP*DTP*DCP*DTP*DCP*DTP*DGP*DAP*DTP*DTP*DTP*DAP*DTP*DC)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 128.882Å b: 30.37Å c: 75.648Å
α: 90° β: 93.82° γ: 90°
R-values:
R R work R free
0.276 0.276 0.298
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation.
Ho et al. (2009)
17 more

12 mentions without citation

CRISPR-Mediated Epigenome Editing.
Enríquez P. (2016)
11 more