› 3dow

X-ray diffraction
2.3Å resolution

Complex structure of GABA type A receptor associated protein and its binding epitope on calreticulin

Released:
DOI: 10.2210/pdb3dow/pdb
PDB entry 3dow coloured by chain, front view.
PDB entry 3dow coloured by chain, side view.
PDB entry 3dow coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural framework of the GABARAP-calreticulin interface--implications for substrate binding to endoplasmic reticulum chaperones.
Thielmann Y, Weiergräber OH, Mohrlüder J, Willbold D
FEBS J 276 1140-52 (2009)
PMID: 19154346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131848 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gamma-aminobutyric acid receptor-associated protein Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 14.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O95166 (Residues: 1-117; Coverage: 100%)
Gene names: FLC3B, GABARAP, HT004
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Calreticulin Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P27797 (Residues: 195-205; Coverage: 3%)
Gene names: CALR, CRTC

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I23
Unit cell:
a: 97.043Å b: 97.043Å c: 97.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.27
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 review citations

Atg8-family interacting motif crucial for selective autophagy.
Noda et al. (2010)
6 more

5 mentions without citation

Interaction of Bcl-2 with the autophagy-related GABAA receptor-associated protein (GABARAP): biophysical characterization and functional implications.
Ma et al. (2013)
4 more