› 3dsf

X-ray diffraction
2.8Å resolution

Crystal structure of anti-osteopontin antibody 23C3 in complex with W43A mutated epitope peptide

Released:
DOI: 10.2210/pdb3dsf/pdb
PDB entry 3dsf coloured by chain, front view.
PDB entry 3dsf coloured by chain, side view.
PDB entry 3dsf coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of recognition of human osteopontin by 23C3, a potential therapeutic antibody for treatment of rheumatoid arthritis.
Du J, Hou S, Zhong C, Lai Z, Yang H, Dai J, Zhang D, Wang H, Guo Y, Ding J
J Mol Biol 382 835-42 (2008)
PMID: 18694758

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233978 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Fab fragment of anti-osteopontin antibody 23C3, Light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.07 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment of anti-osteopontin antibody 23C3, Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 23.13 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Osteopontin Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10451 (Residues: 40-51; Coverage: 4%)
Gene names: BNSP, OPN, PSEC0156, SPP1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 41.73Å b: 92.058Å c: 122.104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.241 0.297
Expression system: Not provided

Quick links

Citations

1 review citation

Thrombin Cleavage of Osteopontin and the Host Anti-Tumor Immune Response.
Leung et al. (2023)
5 other articles

3 mentions without citation

Molecular basis of recognition of human osteopontin by 23C3, a potential therapeutic antibody for treatment of rheumatoid arthritis.
Du et al. (2008)
2 more