› 3dtq

X-ray diffraction
2.5Å resolution

Engineered human lipocalin 2 with specificity for Y-DTPA, apo-form

Released:
DOI: 10.2210/pdb3dtq/pdb
PDB entry 3dtq coloured by chain, front view.
PDB entry 3dtq coloured by chain, side view.
PDB entry 3dtq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2.
Kim HJ, Eichinger A, Skerra A
J Am Chem Soc 131 3565-76 (2009)
PMID: 19227970

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil gelatinase-associated lipocalin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 186 amino acids
Theoretical weight: 21.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 113.593Å b: 113.593Å c: 119.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.262 0.291
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Engineered protein scaffolds as next-generation antibody therapeutics.
Gebauer et al. (2009)
6 more

2 mentions without citation

The major cockroach allergen Bla g 4 binds tyramine and octopamine.
Offermann et al. (2014)
1 more