› 3dwb

X-ray diffraction
2.38Å resolution

structure of human ECE-1 complexed with phosphoramidon

Released:
DOI: 10.2210/pdb3dwb/pdb
PDB entry 3dwb coloured by chain, front view.
PDB entry 3dwb coloured by chain, side view.
PDB entry 3dwb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human endothelin-converting enzyme I complexed with phosphoramidon.
Schulz H, Dale GE, Karimi-Nejad Y, Oefner C
J Mol Biol 385 178-87 (2009)
PMID: 18992253

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the -Trp(21)-|-Val- bond in big endothelin to form endothelin 1.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endothelin-converting enzyme 1 Chain: A
Molecule details ›
Chain: A
Length: 670 amino acids
Theoretical weight: 76.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42892 (Residues: 101-770; Coverage: 87%)
Gene name: ECE1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand 5HD 1 x 5HD
Ligand RDF 1 x RDF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P6122
Unit cell:
a: 120.88Å b: 120.88Å c: 192.346Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.262 0.345
Expression system: Escherichia coli

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Citations

3 review citations

Amyloid cascade hypothesis: Pathogenesis and therapeutic strategies in Alzheimer's disease.
Barage et al. (2015)
2 more

9 mentions without citation

Predicting new indications for approved drugs using a proteochemometric method.
Dakshanamurthy et al. (2012)
8 more