› 3e04

X-ray diffraction
1.95Å resolution

Crystal structure of human fumarate hydratase

Released:
DOI: 10.2210/pdb3e04/pdb
PDB entry 3e04 coloured by chain, front view.
PDB entry 3e04 coloured by chain, side view.
PDB entry 3e04 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kavanagh KL, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 490 amino acids
Theoretical weight: 52.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07954 (Residues: 44-510; Coverage: 92%)
Gene name: FH
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 188.483Å b: 188.483Å c: 114.632Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.244
Expression system: Escherichia coli

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