› 3e4c

X-ray diffraction
2.05Å resolution

Procaspase-1 zymogen domain crystal structure

Released:
DOI: 10.2210/pdb3e4c/pdb
PDB entry 3e4c coloured by chain, front view.
PDB entry 3e4c coloured by chain, side view.
PDB entry 3e4c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of procaspase-1 zymogen domain reveals insight into inflammatory caspase autoactivation.
Elliott JM, Rouge L, Wiesmann C, Scheer JM
J Biol Chem 284 6546-53 (2009)
PMID: 19117953

Function and Biology Details

Reaction catalysed: 
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-1 subunit p20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 33.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29466 (Residues: 104-404; Coverage: 75%)
Gene names: CASP1, IL1BC, IL1BCE
Sequence domains: Caspase domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 56.1Å b: 58.004Å c: 60.12Å
α: 105.95° β: 119.6° γ: 92.77°
R-values:
R R work R free
0.204 0.2 0.263
Expression system: Escherichia coli

Quick links

Citations

19 review citations

A Mechanistic Understanding of Pyroptosis: The Fiery Death Triggered by Invasive Infection.
Liu et al. (2017)
18 more

9 mentions without citation

Small Molecule Active Site Directed Tools for Studying Human Caspases.
Poreba et al. (2015)
8 more