› 3e95

X-ray diffraction
2.5Å resolution

Crystal Structure of the Plasmodium Falciparum ubiquitin conjugating enzyme complex, PfUBC13-PfUev1a

Released:
DOI: 10.2210/pdb3e95/pdb
PDB entry 3e95 coloured by chain, front view.
PDB entry 3e95 coloured by chain, side view.
PDB entry 3e95 coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Entry authors: Wernimont AK, Lam A, Ali A, Brokx S, Lin YH, Zhao Y, Lew J, Ravichandran M, Wasney G, Vedadi M, Kozieradzki I, Schapira M, Bochkarev A, Wilkstrom M, BOuntra C, Arrowsmith CH, Edwards AM, Hui R, Qiu W, Brand VB, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132181 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UBC core domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17.27 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I3J4 (Residues: 3-152; Coverage: 99%)
Gene name: PF3D7_0527100
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
UBC core domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 158 amino acids
Theoretical weight: 18.13 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: O97241 (Residues: 1-140; Coverage: 99%)
Gene name: PF3D7_0305700
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand UNX 5 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 154.856Å b: 154.856Å c: 81.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.248 0.258
Expression system: Escherichia coli

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