› 3e9l

X-ray diffraction
1.95Å resolution

Crystal Structure of Human Prp8, Residues 1755-2016

Released:
DOI: 10.2210/pdb3e9l/pdb
PDB entry 3e9l coloured by chain, front view.
PDB entry 3e9l coloured by chain, side view.
PDB entry 3e9l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of an RNase H domain at the heart of the spliceosome.
Pena V, Rozov A, Fabrizio P, Lührmann R, Wahl MC
EMBO J 27 2929-40 (2008)
PMID: 18843295

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-processing-splicing factor 8 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P2Q9 (Residues: 1760-2016; Coverage: 11%)
Gene names: PRPC8, PRPF8
Sequence domains: PRP8 domain IV core
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P3221
Unit cell:
a: 83.426Å b: 83.426Å c: 96.263Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.205 0.248
Expression system: Escherichia coli

Quick links

Citations

19 review citations

The spliceosome: design principles of a dynamic RNP machine.
Wahl et al. (2009)
18 more

4 mentions without citation

Structure and function of an RNase H domain at the heart of the spliceosome.
Pena et al. (2008)
3 more