› 3ea5

X-ray diffraction
2.5Å resolution

Kap95p Binding Induces the Switch Loops of RanGDP to adopt the GTP-bound Conformation: Implications for Nuclear Import Complex Assembly Dynamics

Released:
DOI: 10.2210/pdb3ea5/pdb
PDB entry 3ea5 coloured by chain, front view.
PDB entry 3ea5 coloured by chain, side view.
PDB entry 3ea5 coloured by chain, top view.
Source organisms:
Primary publication:
Kap95p binding induces the switch loops of RanGDP to adopt the GTP-bound conformation: implications for nuclear import complex assembly dynamics.
Forwood JK, Lonhienne TG, Marfori M, Robin G, Meng W, Guncar G, Liu SM, Stewart M, Carroll BJ, Kobe B
J Mol Biol 383 772-82 (2008)
PMID: 18708071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158721 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein Ran Chains: A, C
Molecule details ›
Chains: A, C
Length: 216 amino acids
Theoretical weight: 24.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Importin subunit beta-1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 861 amino acids
Theoretical weight: 94.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06142 (Residues: 1-861; Coverage: 100%)
Gene names: KAP95, L8300.15, YLR347C
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 110.85Å b: 127.81Å c: 171.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Nuclear import by karyopherin-βs: recognition and inhibition.
Chook et al. (2011)
2 more

2 mentions without citation

Domain-Based Protein Docking with Extremely Large Conformational Changes.
Christoffer et al. (2022)
1 more