› 3eba

X-ray diffraction
1.85Å resolution

CAbHul6 FGLW mutant (humanized) in complex with human lysozyme

Released:
DOI: 10.2210/pdb3eba/pdb
PDB entry 3eba coloured by chain, front view.
PDB entry 3eba coloured by chain, side view.
PDB entry 3eba coloured by chain, top view.
Source organisms:
Primary publication:
General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold.
Vincke C, Loris R, Saerens D, Martinez-Rodriguez S, Muyldermans S, Conrath K
J Biol Chem 284 3273-3284 (2009)
PMID: 19010777

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234050 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CAbHul6 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.85 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Lysozyme C Chain: B
Molecule details ›
Chain: B
Length: 130 amino acids
Theoretical weight: 14.72 KDa
Source organism: Homo sapiens
Expression system: Aspergillu niger
UniProt:
  • Canonical: P61626 (Residues: 19-148; Coverage: 100%)
Gene names: LYZ, LZM
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P61
Unit cell:
a: 63.67Å b: 63.67Å c: 119.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.255
Expression systems:
  • Escherichia coli
  • Aspergillu niger

Quick links

Citations

98 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
97 more

2 mentions without citation

Structural Basis of the Recruitment of Ubiquitin-specific Protease USP15 by Spliceosome Recycling Factor SART3.
Zhang et al. (2016)
1 more