› 3ee0

X-ray diffraction
2.75Å resolution

Crystal Structure of the W215A/E217A Mutant of Human Thrombin (space group P2(1)2(1)2(1))

Released:
DOI: 10.2210/pdb3ee0/pdb
PDB entry 3ee0 coloured by chain, front view.
PDB entry 3ee0 coloured by chain, side view.
PDB entry 3ee0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Gandhi PS, Page MJ, Chen Z, Bush-Pelc L, Di Cera E

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133055 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Sequence domains: Thrombin light chain
Thrombin heavy chain Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 29.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 40.142Å b: 60.007Å c: 120.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.323
Expression system: Cricetulus griseus

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