› 3egh

X-ray diffraction
2Å resolution

Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1), the PP1 binding and PDZ domains of Spinophilin and the small natural molecular toxin Nodularin-R

Released:
DOI: 10.2210/pdb3egh/pdb
PDB entry 3egh coloured by chain, front view.
PDB entry 3egh coloured by chain, side view.
PDB entry 3egh coloured by chain, top view.
Source organisms:
Primary publication:
Spinophilin directs protein phosphatase 1 specificity by blocking substrate binding sites.
Ragusa MJ, Dancheck B, Critton DA, Nairn AC, Page R, Peti W
Nat Struct Mol Biol 17 459-64 (2010)
PMID: 20305656

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-128667 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 37.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 7-330; Coverage: 98%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Neurabin-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 170 amino acids
Theoretical weight: 18.57 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35274 (Residues: 417-583; Coverage: 20%)
Gene names: Ppp1r9b, Spino
Sequence domains:
Structure domains: Pdz3 Domain
nodularin R Chains: E, F
Molecule details ›
Chains: E, F
Length: 5 amino acids
Theoretical weight: 844 Da
Source organism: Nodularia spumigena
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand MN 4 x MN
3 modified residues:
Ligand ACB 2 x ACB
Ligand FGA 2 x FGA
Ligand MDH 2 x MDH

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 119.409Å b: 84.426Å c: 109.314Å
α: 90° β: 93.58° γ: 90°
R-values:
R R work R free
0.195 0.192 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

33 review citations

Toxin-antitoxin systems in bacterial growth arrest and persistence.
Page et al. (2016)
32 more

6 mentions without citation

Structural basis for protein phosphatase 1 regulation and specificity.
Peti et al. (2013)
5 more