3ejz

X-ray diffraction
2.9Å resolution

Structure of E203V mutant E.coli Cl-/H+ exchanger, CLC-ec1

Released:
Source organisms:
Primary publication:
Intracellular proton-transfer mutants in a CLC Cl-/H+ exchanger.
J Gen Physiol 133 131-8 (2009)
PMID: 19139174

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
homo dimer
hetero dimer
PDBe Complex ID:
PDB-CPX-208563 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab fragment, Heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 221 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-104; Coverage: 97%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 231.259Å b: 96.415Å c: 170.15Å
α: 90° β: 131.78° γ: 90°
R-values:
R R work R free
0.253 0.252 0.281
Expression system: Escherichia coli