› 3ek7

X-ray diffraction
1.85Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.7 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11799 (Residues: 1730-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-144, 191-192; Coverage: 69%)
  • Canonical: P0DP29 (Residues: 3-42, 45-149; Coverage: 84%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, GFP, Mylk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 126.131Å b: 47.31Å c: 68.94Å
α: 90° β: 100.48° γ: 90°
R-values:
R R work R free
0.191 0.189 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.
Rodriguez et al. (2017)
37 more

4 mentions without citation

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Akerboom et al. (2013)
3 more