› 3elb

X-ray diffraction
2Å resolution

Human CTP: Phosphoethanolamine Cytidylyltransferase in complex with CMP

Released:
DOI: 10.2210/pdb3elb/pdb
PDB entry 3elb coloured by chain, front view.
PDB entry 3elb coloured by chain, side view.
PDB entry 3elb coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Karlberg T, Welin M, Andersson J, Arrowsmith CH, Berglund H, Bountra C, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Johansson A, Johansson I, Kotenyova T, Lehtio L, Moche M, Nilsson ME, Nordlund P, Nyman T, Persson C, Sagemark J, Thorsell AG, Tresaugues L, Van Den Berg S, Weigelt J, Wikstrom M, Wisniewska M, Schuler H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ethanolamine-phosphate cytidylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 39.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99447 (Residues: 18-356; Coverage: 87%)
Gene name: PCYT2
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand C5P 1 x C5P
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 74.179Å b: 74.179Å c: 139.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.242
Expression system: Escherichia coli

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