› 3eo3

X-ray diffraction
2.84Å resolution

Crystal structure of the N-acetylmannosamine kinase domain of human GNE protein

Released:
DOI: 10.2210/pdb3eo3/pdb
PDB entry 3eo3 coloured by chain, front view.
PDB entry 3eo3 coloured by chain, side view.
PDB entry 3eo3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the N-acetylmannosamine kinase domain of GNE.
Tong Y, Tempel W, Nedyalkova L, Mackenzie F, Park HW
PLoS One 4 e7165 (2009)
PMID: 19841673

Function and Biology Details

Reactions catalysed: 
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate
UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 333 amino acids
Theoretical weight: 35.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y223 (Residues: 406-720; Coverage: 44%)
Gene names: GLCNE, GNE, IBM2
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B, APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 127.946Å b: 127.946Å c: 127.247Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.245
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mutation update for GNE gene variants associated with GNE myopathy.
Celeste et al. (2014)
3 more

5 mentions without citation

Crystal structure of the N-acetylmannosamine kinase domain of GNE.
Tong et al. (2009)
4 more