› 3epp

X-ray diffraction
2.41Å resolution

Crystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefungin

Released:
DOI: 10.2210/pdb3epp/pdb
PDB entry 3epp coloured by chain, front view.
PDB entry 3epp coloured by chain, side view.
PDB entry 3epp coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Amaya MF, Zeng H, Loppnau P, Bountra C, Weigelt J, Arrowsmith CH, Edwards AM, Botchkarev A, Min J, Plotnikov AN, Wu H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128838 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mRNA cap guanine-N7 methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 36.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43148 (Residues: 165-476; Coverage: 66%)
Gene names: KIAA0398, RNMT
Sequence domains: mRNA (guanine-N(7))-methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SFG 2 x SFG
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 58.515Å b: 73.65Å c: 147.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.3
Expression system: Escherichia coli

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