› 3eto

X-ray diffraction
2Å resolution

2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR)

Released:
DOI: 10.2210/pdb3eto/pdb
PDB entry 3eto coloured by chain, front view.
PDB entry 3eto coloured by chain, side view.
PDB entry 3eto coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Notch1-negative regulatory region: implications for normal activation and pathogenic signaling in T-ALL.
Gordon WR, Roy M, Vardar-Ulu D, Garfinkel M, Mansour MR, Aster JC, Blacklow SC
Blood 113 4381-90 (2009)
PMID: 19075186

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Notch 1 extracellular truncation Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 26.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1446-1733; Coverage: 10%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand CL 2 x CL
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 169.927Å b: 91.759Å c: 59.844Å
α: 90° β: 109.38° γ: 90°
R-values:
R R work R free
0.213 0.211 0.252
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Canonical and non-canonical Notch ligands.
D'Souza et al. (2010)
40 more

19 mentions without citation

Notch receptor-ligand binding and activation: insights from molecular studies.
Chillakuri et al. (2012)
18 more