› 3evu

X-ray diffraction
1.75Å resolution

Crystal structure of Calcium bound dimeric GCAMP2

Released:
DOI: 10.2210/pdb3evu/pdb
PDB entry 3evu coloured by chain, front view.
PDB entry 3evu coloured by chain, side view.
PDB entry 3evu coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for calcium sensing by GCaMP2.
Wang Q, Shui B, Kotlikoff MI, Sondermann H
Structure 16 1817-27 (2008)
PMID: 19081058

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase, smooth muscle, deglutamylated form Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.7 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P11799 (Residues: 1730-1763; Coverage: 2%)
  • Canonical: P42212 (Residues: 2-144, 191-192; Coverage: 61%)
  • Canonical: P0DP29 (Residues: 3-42, 45-148; Coverage: 97%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1, GFP, Mylk
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 127.936Å b: 47.31Å c: 69.642Å
α: 90° β: 100.7° γ: 90°
R-values:
R R work R free
0.164 0.161 0.197
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Genetically encoded indicators of neuronal activity.
Lin et al. (2016)
18 more

1 mention without citation

An expanded palette of genetically encoded Ca²⁺ indicators.
Zhao et al. (2011)