› 3exj

X-ray diffraction
2Å resolution

Crystal Structure of a p53 Core Tetramer Bound to DNA

Released:
DOI: 10.2210/pdb3exj/pdb
PDB entry 3exj coloured by chain, front view.
PDB entry 3exj coloured by chain, side view.
PDB entry 3exj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of a p53 core tetramer bound to DNA.
Malecka KA, Ho WC, Marmorstein R
Oncogene 28 325-33 (2009)
PMID: 18978813

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116221 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 197 amino acids
Theoretical weight: 22.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02340 (Residues: 96-292; Coverage: 51%)
Gene names: P53, Tp53, Trp53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
5'-D(P*DGP*DAP*DGP*DCP*DAP*DTP*DGP*DCP*DTP*DCP*DA)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*DTP*DTP*DGP*DAP*DGP*DCP*DAP*DTP*DGP*DCP*DTP*DC)-3' Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 114.74Å b: 68.016Å c: 75.162Å
α: 90° β: 111.12° γ: 90°
R-values:
R R work R free
0.225 0.225 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

The tumor suppressor p53: from structures to drug discovery.
Joerger et al. (2010)
10 more

14 mentions without citation

Good Cop, Bad Cop: Defining the Roles of Δ40p53 in Cancer and Aging.
Steffens Reinhardt et al. (2020)
13 more