› 3f31

X-ray diffraction
2.3Å resolution

Crystal Structure of the N-terminal region of AlphaII-spectrin Tetramerization Domain

Released:
DOI: 10.2210/pdb3f31/pdb
PDB entry 3f31 coloured by chain, front view.
PDB entry 3f31 coloured by chain, side view.
PDB entry 3f31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the nonerythroid alpha-spectrin tetramerization site reveals differences between erythroid and nonerythroid spectrin tetramer formation.
Mehboob S, Song Y, Witek M, Long F, Santarsiero BD, Johnson ME, Fung LW
J Biol Chem 285 14572-84 (2010)
PMID: 20228407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 17.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13813 (Residues: 1-147; Coverage: 6%)
Gene names: NEAS, SPTA2, SPTAN1
Sequence domains: Spectrin repeat
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 57.72Å b: 71.46Å c: 80.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.214 0.28
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Spectrins: a structural platform for stabilization and activation of membrane channels, receptors and transporters.
Machnicka et al. (2014)
15 other articles

4 mentions without citation

Crystal structure of the nonerythroid alpha-spectrin tetramerization site reveals differences between erythroid and nonerythroid spectrin tetramer formation.
Mehboob et al. (2010)
3 more