› 3f97

X-ray diffraction
1.7Å resolution

Crystal structure of human plasma platelet activating factor acetylhydrolase covalently inhibited by soman

Released:
DOI: 10.2210/pdb3f97/pdb
PDB entry 3f97 coloured by chain, front view.
PDB entry 3f97 coloured by chain, side view.
PDB entry 3f97 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes.
Samanta U, Kirby SD, Srinivasan P, Cerasoli DM, Bahnson BJ
Biochem Pharmacol 78 420-9 (2009)
PMID: 19394314

Function and Biology Details

Reaction catalysed: 
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Platelet-activating factor acetylhydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 43.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13093 (Residues: 47-429; Coverage: 91%)
Gene names: PAFAH, PLA2G7
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand GD7 2 x GD7
Ligand SO4 1 x SO4
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 116.084Å b: 82.697Å c: 96.692Å
α: 90° β: 115.33° γ: 90°
R-values:
R R work R free
0.183 0.182 0.212
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Review of four major distinct types of human phospholipase A2.
Vasquez et al. (2018)
2 more

3 mentions without citation

Lipoprotein-associated phospholipase A2: The story continues.
Huang et al. (2020)
2 more