› 3fcx

X-ray diffraction
1.5Å resolution

Crystal structure of human esterase D

Released:
DOI: 10.2210/pdb3fcx/pdb
PDB entry 3fcx coloured by chain, front view.
PDB entry 3fcx coloured by chain, side view.
PDB entry 3fcx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human esterase D: a potential genetic marker of retinoblastoma.
Wu D, Li Y, Song G, Zhang D, Shaw N, Liu ZJ
FASEB J 23 1441-6 (2009)
PMID: 19126594

Function and Biology Details

Reactions catalysed: 
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
S-formylglutathione + H(2)O = glutathione + formate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-formylglutathione hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10768 (Residues: 1-282; Coverage: 100%)
Gene name: ESD
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 51.542Å b: 70.724Å c: 65.014Å
α: 90° β: 108.84° γ: 90°
R-values:
R R work R free
0.179 0.176 0.196
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

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Kakugawa et al. (2015)
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8 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
7 more