› 3fep

X-ray diffraction
2.6Å resolution

Crystal structure of the R132K:R111L:L121E:R59W-CRABPII mutant complexed with a synthetic ligand (merocyanin) at 2.60 angstrom resolution.

Released:
DOI: 10.2210/pdb3fep/pdb
PDB entry 3fep coloured by chain, front view.
PDB entry 3fep coloured by chain, side view.
PDB entry 3fep coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
"Turn-on" protein fluorescence: in situ formation of cyanine dyes.
Yapici I, Lee KS, Berbasova T, Nosrati M, Jia X, Vasileiou C, Wang W, Santos EM, Geiger JH, Borhan B
J Am Chem Soc 137 1073-80 (2015)
PMID: 25534273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand LMC 1 x LMC
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 55.808Å b: 55.808Å c: 108.177Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.232 0.293
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Cyanine polyene reactivity: scope and biomedical applications.
Gorka et al. (2015)
8 more

1 mention without citation

"Turn-on" protein fluorescence: in situ formation of cyanine dyes.
Yapici et al. (2015)