› 3fmo

X-ray diffraction
2.51Å resolution

Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19

Released:
DOI: 10.2210/pdb3fmo/pdb
PDB entry 3fmo coloured by chain, front view.
PDB entry 3fmo coloured by chain, side view.
PDB entry 3fmo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19.
Napetschnig J, Kassube SA, Debler EW, Wong RW, Blobel G, Hoelz A
Proc Natl Acad Sci U S A 106 3089-94 (2009)
PMID: 19208808

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152988 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear pore complex protein Nup214 Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 49.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35658 (Residues: 1-450; Coverage: 22%)
Gene names: CAIN, CAN, KIAA0023, NUP214
ATP-dependent RNA helicase DDX19B Chain: B
Molecule details ›
Chain: B
Length: 300 amino acids
Theoretical weight: 33.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UMR2 (Residues: 1-300; Coverage: 63%)
Gene names: DBP5, DDX19, DDX19B, TDBP
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 59.484Å b: 115.392Å c: 143.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.242
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The structure of the nuclear pore complex.
Hoelz et al. (2011)
14 more

3 mentions without citation

Mechanisms of nuclear mRNA export: A structural perspective.
Xie et al. (2019)
2 more