› 3frs

X-ray diffraction
2.61Å resolution

Structure of human IST1(NTD) (residues 1-189)(p43212)

Released:
DOI: 10.2210/pdb3frs/pdb
PDB entry 3frs coloured by chain, front view.
PDB entry 3frs coloured by chain, side view.
PDB entry 3frs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for ESCRT-III protein autoinhibition.
Bajorek M, Schubert HL, McCullough J, Langelier C, Eckert DM, Stubblefield WM, Uter NT, Myszka DG, Hill CP, Sundquist WI
Nat Struct Mol Biol 16 754-62 (2009)
PMID: 19525971

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IST1 homolog Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53990 (Residues: 5-189; Coverage: 51%)
Gene names: IST1, KIAA0174
Sequence domains: Regulator of Vps4 activity in the MVB pathway
Structure domains: Vacuolar protein sorting-associated protein Ist1

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF, SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 57.18Å b: 57.18Å c: 157.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.259 0.298
Expression system: Escherichia coli

Quick links

Citations

40 review citations

The ESCRT pathway.
Henne et al. (2011)
39 more

2 mentions without citation

Dividing the Archaeal Way: The Ancient Cdv Cell-Division Machinery.
Caspi et al. (2018)
1 more