› 3fwv

X-ray diffraction
2.2Å resolution

Crystal Structure of a Redesigned TPR Protein, T-MOD(VMY), in Complex with MEEVF Peptide

Released:
DOI: 10.2210/pdb3fwv/pdb
PDB entry 3fwv coloured by chain, front view.
PDB entry 3fwv coloured by chain, side view.
PDB entry 3fwv coloured by chain, top view.
Source organisms:
Primary publication:
Redesign of a protein-peptide interaction: characterization and applications.
Jackrel ME, Valverde R, Regan L
Protein Sci 18 762-74 (2009)
PMID: 19309728

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140048 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stress-induced-phosphoprotein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 15.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31948 (Residues: 223-349; Coverage: 23%)
Gene name: STIP1
Sequence domains:
Structure domains: Tetratricopeptide repeat domain
Heat shock protein HSP 90-beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 680 Da
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P08238 (Residues: 720-724; Coverage: 1%)
Gene names: HSP90AB1, HSP90B, HSPC2, HSPC3, HSPCB

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 37.604Å b: 66.599Å c: 48.622Å
α: 90° β: 107.21° γ: 90°
R-values:
R R work R free
0.187 0.184 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Structural and functional discussion of the tetra-trico-peptide repeat, a protein interaction module.
Zeytuni et al. (2012)
4 more

2 mentions without citation

Redesign of a protein-peptide interaction: characterization and applications.
Jackrel et al. (2009)
1 more