› 3fxy

X-ray diffraction
2Å resolution

Acidic Mammalian Chinase, Catalytic Domain

Released:
DOI: 10.2210/pdb3fxy/pdb
PDB entry 3fxy coloured by chain, front view.
PDB entry 3fxy coloured by chain, side view.
PDB entry 3fxy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Triad of polar residues implicated in pH specificity of acidic mammalian chitinase.
Olland AM, Strand J, Presman E, Czerwinski R, Joseph-McCarthy D, Krykbaev R, Schlingmann G, Chopra R, Lin L, Fleming M, Kriz R, Stahl M, Somers W, Fitz L, Mosyak L
Protein Sci 18 569-78 (2009)
PMID: 19241384

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acidic mammalian chitinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 395 amino acids
Theoretical weight: 44.2 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q9BZP6 (Residues: 22-408; Coverage: 85%)
Gene name: CHIA
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 69.941Å b: 92.36Å c: 111.669Å
α: 90° β: 95.92° γ: 90°
R-values:
R R work R free
0.199 0.197 0.228
Expression system: Cricetulus griseus

Quick links

Citations

5 review citations

Chitins and chitosans as immunoadjuvants and non-allergenic drug carriers.
Muzzarelli RA. (2010)
4 more

10 mentions without citation

Differential enzymatic activity of common haplotypic versions of the human acidic Mammalian chitinase protein.
Seibold et al. (2009)
9 more